Epigenome Editing, Methods and Protocols

2018-06-01

EpiPredict researchers describe state-of-the-art methods to study epigenetic reprogramming of cell, i.e. illustrating ChIP-seq laboratory protocol and downstream data analysis (Pattern et al. Methods Mol. Biol. 2018: 1767:271-288); establishment of cell lines stably expressing dCas9 fusions to address kinetics of epigenetic editing (Goubert et al. Methods Mol. Biol. 2018: 1767: 395-415); cell-to-cell transcription variability as measured by single-molecule RNA FISH to detect epigenetic state switching (Beckman et al. Methods Mol. Biol. 2018: 1767: 385-393) in Epigenome Editing Methods and Protocols edited by prof. Marianne Rots and prof. Albert Jeltsch. This edition of the highly successful Methods in Molecular Biology series includes concepts and applications of epigenome editing by leading scientists in the field, covering design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.